Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2667604..2668180 Replicon chromosome
Accession NZ_CP060950
Organism Escherichia coli strain EC9

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag H9196_RS12835 Protein ID WP_001350372.1
Coordinates 2667604..2667891 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag H9196_RS12840 Protein ID WP_001402821.1
Coordinates 2667878..2668180 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9196_RS12820 (2663186) 2663186..2664568 - 1383 WP_023155808.1 restriction endonuclease subunit S -
H9196_RS12825 (2664558) 2664558..2666177 - 1620 WP_001029742.1 class I SAM-dependent DNA methyltransferase -
H9196_RS12830 (2666241) 2666241..2667407 - 1167 WP_001672354.1 restriction endonuclease -
H9196_RS12835 (2667604) 2667604..2667891 + 288 WP_001350372.1 BrnT family toxin Toxin
H9196_RS12840 (2667878) 2667878..2668180 + 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
H9196_RS12845 (2668214) 2668214..2669170 - 957 WP_001295525.1 GTPase -
H9196_RS12850 (2669181) 2669181..2669384 - 204 WP_000467859.1 YbdD/YjiX family protein -
H9196_RS12855 (2669434) 2669434..2671584 - 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
H9196_RS12860 (2671977) 2671977..2672489 - 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T171693 WP_001350372.1 NZ_CP060950:2667604-2667891 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171693 NZ_CP078030:c2775-2485 [Acinetobacter variabilis]
ATGGTTAAAATAGCTGATCTACCTAGCTTTGACATGGCAGAAGCACTTAAGAGTGAAGAAGATATTGTAATGTATCTTAA
TATGGTTCTTGAAGAGAATGACCCTGCTGAACTAGCTCATGCACTTGGTGTAATTGCTAAAGCTCGTGGTATGACTCAAA
TCGCTAAAGAAGCAGGAATTGGACGTGAAGCACTTTACAAAGCACTCCGTCAAGATTCAGCACCTCGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGTCTAAAACTAACTGTGCAGAATGCATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT171693 WP_001402821.1 NZ_CP060950:2667878-2668180 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171693 NZ_CP078030:c3075-2779 [Acinetobacter variabilis]
ATGACCGAGATTAAACGTCTCCCTGAATTTGATGAATGGCTAGATAGCATTAAAGACAGTATGACCCGTCTTAAACTGAA
TCGAAGATTAGATAAGGTTCAGCGTGGTAATTGGGGCGATGTAAAGCATCTTCAAGAAGGTGTTTGGGAAATGCGAGAGT
TCTTCGGACCAGGTTGGCGCATGTATTACATCAAACATGGTGATGTAATCATTGTGATGCTTGGAGGTGGTGATAAATCT
ACACAGCAAAAGGATATAGAACGAGCTGTTGATTTATCAAAATCTTTAGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References