Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1085622..1086276 Replicon chromosome
Accession NZ_CP060950
Organism Escherichia coli strain EC9

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H9196_RS05175 Protein ID WP_000244772.1
Coordinates 1085622..1086029 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9196_RS05180 Protein ID WP_000354046.1
Coordinates 1086010..1086276 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9196_RS05155 (1081579) 1081579..1083312 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
H9196_RS05160 (1083318) 1083318..1084028 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9196_RS05165 (1084053) 1084053..1084949 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9196_RS05170 (1085061) 1085061..1085582 + 522 WP_001055874.1 flavodoxin FldB -
H9196_RS05175 (1085622) 1085622..1086029 - 408 WP_000244772.1 protein YgfX Toxin
H9196_RS05180 (1086010) 1086010..1086276 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9196_RS05185 (1086519) 1086519..1087499 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9196_RS05190 (1087576) 1087576..1088235 - 660 WP_000250274.1 hemolysin III family protein -
H9196_RS05195 (1088399) 1088399..1088710 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9196_RS05200 (1088755) 1088755..1090188 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H9196_RS05205 (1090245) 1090245..1090988 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T171687 WP_000244772.1 NZ_CP060950:c1086029-1085622 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171687 NZ_CP078019:c320727-320407 [Acinetobacter junii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171687 WP_000354046.1 NZ_CP060950:c1086276-1086010 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171687 NZ_CP078019:c320414-320142 [Acinetobacter junii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References