Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19383..20026 Replicon plasmid pEC10-3
Accession NZ_CP060948
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H9197_RS23620 Protein ID WP_001034044.1
Coordinates 19610..20026 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H9197_RS23615 Protein ID WP_001261286.1
Coordinates 19383..19613 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS23600 (14520) 14520..14750 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
H9197_RS23605 (14747) 14747..15163 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
H9197_RS23610 (15208) 15208..19002 - 3795 WP_241407768.1 pcar -
H9197_RS23615 (19383) 19383..19613 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9197_RS23620 (19610) 19610..20026 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H9197_RS23625 (20101) 20101..21666 + 1566 WP_001128474.1 AAA family ATPase -
H9197_RS23630 (21651) 21651..22673 + 1023 WP_000361402.1 helicase UvrD -
H9197_RS23640 (23681) 23681..24037 + 357 Protein_28 chromate transporter -
H9197_RS23645 (24047) 24047..24352 + 306 WP_000130000.1 PadR family transcriptional regulator -
H9197_RS23650 (24396) 24396..24461 + 66 Protein_30 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / mph(A) - 1..86240 86240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T171678 WP_001034044.1 NZ_CP060948:19610-20026 [Escherichia coli O157:H7 str. EC10]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T171678 NZ_CP078017:c1456968-1456663 [Alcaligenes aquatilis]
ATGAACACGATCAACCGCAGCGAAACTTTCAGCACCTGGCTAACAGGCTTAAAAGACCTCAAAGCCAGAGCGAAGATCGT
GGTTCGCATCAAACAAGCCAGCCTGGGCAATTTCGGAGACGTAAAGCCAATTAGTGATGGTGTGTATGAAATGCGCATCC
ACTTCGGTCCTGGCTACCGTCTCTACTATGCTCGTGAGGGCCGTGTGGTCTATCTGCTGCTTAGCGGTGGCGACAAGTCC
TCCCAAAAACAAGACATCAAAACTGCAATCGCCATGTGGAAACAAATTCAGGAGGATCAGTCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT171678 WP_001261286.1 NZ_CP060948:19383-19613 [Escherichia coli O157:H7 str. EC10]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT171678 NZ_CP078017:c1456666-1456373 [Alcaligenes aquatilis]
ATGACCACCCTCAAAACAGCCCCCTTCGACGCCGCCGAATATCTTGATAGCGAGGAAGCTATTGCCGAGTACCTCTCGGC
TGCTTTGGAAGAAGACGATCCAGCCTTGTTCCTCTCTGCGCTTGCGGATGTGGTCAAAGCCCGAGGCATGACAAAAATGG
CTAAAGACGCCGGTGTAGGCCGAGAAAGCCTGTACAAGGCTCTTGCTCCGGGCGCCAAGCCACGATATGACACTGTCCTG
AAGTTAGCGCGGGCTGCTGGGGTGAAATTAACGGTAGAGCCGATTCATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References