Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14520..15163 Replicon plasmid pEC10-3
Accession NZ_CP060948
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag H9197_RS23605 Protein ID WP_001034046.1
Coordinates 14747..15163 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag H9197_RS23600 Protein ID WP_001261278.1
Coordinates 14520..14750 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS23575 (10233) 10233..10583 - 351 WP_000493378.1 hypothetical protein -
H9197_RS23580 (11147) 11147..11635 + 489 WP_011254646.1 hypothetical protein -
H9197_RS23585 (11669) 11669..12802 - 1134 WP_000545987.1 DUF3800 domain-containing protein -
H9197_RS23590 (12969) 12969..13742 - 774 WP_000905949.1 hypothetical protein -
H9197_RS23595 (13755) 13755..14255 - 501 WP_000528931.1 HEPN family nuclease -
H9197_RS23600 (14520) 14520..14750 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9197_RS23605 (14747) 14747..15163 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
H9197_RS23610 (15208) 15208..19002 - 3795 WP_241407768.1 pcar -
H9197_RS23615 (19383) 19383..19613 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
H9197_RS23620 (19610) 19610..20026 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fosA3 / mph(A) - 1..86240 86240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T171677 WP_001034046.1 NZ_CP060948:14747-15163 [Escherichia coli O157:H7 str. EC10]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T171677 NZ_CP078017:c1456666-1456373 [Alcaligenes aquatilis]
ATGACCACCCTCAAAACAGCCCCCTTCGACGCCGCCGAATATCTTGATAGCGAGGAAGCTATTGCCGAGTACCTCTCGGC
TGCTTTGGAAGAAGACGATCCAGCCTTGTTCCTCTCTGCGCTTGCGGATGTGGTCAAAGCCCGAGGCATGACAAAAATGG
CTAAAGACGCCGGTGTAGGCCGAGAAAGCCTGTACAAGGCTCTTGCTCCGGGCGCCAAGCCACGATATGACACTGTCCTG
AAGTTAGCGCGGGCTGCTGGGGTGAAATTAACGGTAGAGCCGATTCATGCTTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT171677 WP_001261278.1 NZ_CP060948:14520-14750 [Escherichia coli O157:H7 str. EC10]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT171677 NZ_CP078017:c1456968-1456663 [Alcaligenes aquatilis]
ATGAACACGATCAACCGCAGCGAAACTTTCAGCACCTGGCTAACAGGCTTAAAAGACCTCAAAGCCAGAGCGAAGATCGT
GGTTCGCATCAAACAAGCCAGCCTGGGCAATTTCGGAGACGTAAAGCCAATTAGTGATGGTGTGTATGAAATGCGCATCC
ACTTCGGTCCTGGCTACCGTCTCTACTATGCTCGTGAGGGCCGTGTGGTCTATCTGCTGCTTAGCGGTGGCGACAAGTCC
TCCCAAAAACAAGACATCAAAACTGCAATCGCCATGTGGAAACAAATTCAGGAGGATCAGTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References