Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1985..2544 Replicon plasmid pEC10-2
Accession NZ_CP060947
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name relE Uniprot ID U9YB49
Locus tag H9197_RS23020 Protein ID WP_000421259.1
Coordinates 2269..2544 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID U9Y625
Locus tag H9197_RS23015 Protein ID WP_001178087.1
Coordinates 1985..2269 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS23010 (46) 46..1077 + 1032 WP_000907868.1 plasmid replication initiator RepA -
H9197_RS23015 (1985) 1985..2269 + 285 WP_001178087.1 ribbon-helix-helix domain-containing protein Antitoxin
H9197_RS23020 (2269) 2269..2544 + 276 WP_000421259.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H9197_RS23025 (2650) 2650..2853 + 204 Protein_3 hypothetical protein -
H9197_RS23030 (3123) 3123..3389 - 267 WP_000627832.1 hypothetical protein -
H9197_RS23035 (3389) 3389..3883 - 495 WP_015058884.1 hypothetical protein -
H9197_RS23040 (3939) 3939..4541 - 603 WP_000521606.1 ProQ/FINO family protein -
H9197_RS23045 (4717) 4717..5769 - 1053 WP_000655519.1 hypothetical protein -
H9197_RS23050 (5982) 5982..6572 - 591 WP_001401589.1 hypothetical protein -
H9197_RS23055 (6572) 6572..6826 - 255 WP_000343106.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..87111 87111


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10736.45 Da        Isoelectric Point: 9.9324

>T171672 WP_000421259.1 NZ_CP060947:2269-2544 [Escherichia coli O157:H7 str. EC10]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T171672 NZ_CP078015:c2649439-2649338 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10488.92 Da        Isoelectric Point: 5.6558

>AT171672 WP_001178087.1 NZ_CP060947:1985-2269 [Escherichia coli O157:H7 str. EC10]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT171672 NZ_CP078015:2649215-2649393 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCG
AAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YB49

Antitoxin

Source ID Structure
AlphaFold DB U9Y625

References