Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4324998..4325616 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9197_RS20835 Protein ID WP_001291435.1
Coordinates 4324998..4325216 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9197_RS20840 Protein ID WP_000344800.1
Coordinates 4325242..4325616 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS20800 (4320288) 4320288..4320860 + 573 WP_000779826.1 YbaY family lipoprotein -
H9197_RS20805 (4320891) 4320891..4321202 - 312 WP_000409911.1 MGMT family protein -
H9197_RS20815 (4321581) 4321581..4321934 + 354 WP_000878140.1 DUF1428 domain-containing protein -
H9197_RS20820 (4321976) 4321976..4323526 - 1551 WP_024166621.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9197_RS20825 (4323690) 4323690..4324160 - 471 WP_000136192.1 YlaC family protein -
H9197_RS20830 (4324276) 4324276..4324827 - 552 WP_000102556.1 maltose O-acetyltransferase -
H9197_RS20835 (4324998) 4324998..4325216 - 219 WP_001291435.1 HHA domain-containing protein Toxin
H9197_RS20840 (4325242) 4325242..4325616 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9197_RS20845 (4326162) 4326162..4329311 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9197_RS20850 (4329334) 4329334..4330527 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171670 WP_001291435.1 NZ_CP060946:c4325216-4324998 [Escherichia coli O157:H7 str. EC10]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171670 NZ_CP078015:600971-601087 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171670 WP_000344800.1 NZ_CP060946:c4325616-4325242 [Escherichia coli O157:H7 str. EC10]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171670 NZ_CP078015:c601203-600998 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References