Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3729324..3729900 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag H9197_RS17985 Protein ID WP_001350372.1
Coordinates 3729324..3729611 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag H9197_RS17990 Protein ID WP_001402821.1
Coordinates 3729598..3729900 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS17970 (3724906) 3724906..3726288 - 1383 WP_023155808.1 restriction endonuclease subunit S -
H9197_RS17975 (3726278) 3726278..3727897 - 1620 WP_001029742.1 class I SAM-dependent DNA methyltransferase -
H9197_RS17980 (3727961) 3727961..3729127 - 1167 WP_001672354.1 restriction endonuclease -
H9197_RS17985 (3729324) 3729324..3729611 + 288 WP_001350372.1 BrnT family toxin Toxin
H9197_RS17990 (3729598) 3729598..3729900 + 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
H9197_RS17995 (3729934) 3729934..3730890 - 957 WP_001295525.1 GTPase -
H9197_RS18000 (3730901) 3730901..3731104 - 204 WP_000467859.1 YbdD/YjiX family protein -
H9197_RS18005 (3731154) 3731154..3733304 - 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
H9197_RS18010 (3733697) 3733697..3734209 - 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T171668 WP_001350372.1 NZ_CP060946:3729324-3729611 [Escherichia coli O157:H7 str. EC10]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171668 NZ_CP078015:c304861-304766 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT171668 WP_001402821.1 NZ_CP060946:3729598-3729900 [Escherichia coli O157:H7 str. EC10]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171668 NZ_CP078015:304666-304731 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References