Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3591548..3592143 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag H9197_RS17300 Protein ID WP_000239579.1
Coordinates 3591793..3592143 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag H9197_RS17295 Protein ID WP_001223208.1
Coordinates 3591548..3591799 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS17285 (3587212) 3587212..3590991 + 3780 WP_000060921.1 autotransporter assembly complex protein TamB -
H9197_RS17290 (3590994) 3590994..3591335 + 342 WP_001219160.1 gamma-glutamylcyclotransferase -
H9197_RS17295 (3591548) 3591548..3591799 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
H9197_RS17300 (3591793) 3591793..3592143 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
H9197_RS17305 (3592223) 3592223..3592753 - 531 WP_000055070.1 inorganic diphosphatase -
H9197_RS17310 (3593063) 3593063..3594019 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
H9197_RS17315 (3594159) 3594159..3595661 + 1503 WP_000205800.1 sugar ABC transporter ATP-binding protein -
H9197_RS17320 (3595675) 3595675..3596697 + 1023 WP_001296689.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T171667 WP_000239579.1 NZ_CP060946:3591793-3592143 [Escherichia coli O157:H7 str. EC10]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T171667 NZ_CP078013:c2500396-2500145 [Pseudomonas pergaminensis]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAACGAGCACACGGCATTTACCTGGCCCGAACGCGTGACACTGCT
GCGCCGGCTGGGCTACACACAGATTGAAGGGGCTGGAAGCCGCGTCAAATTCGACATCGGTGACCCCAGTGCAATGATTA
CCTTGCACAAGCCTCACCCGGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAGCAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT171667 WP_001223208.1 NZ_CP060946:3591548-3591799 [Escherichia coli O157:H7 str. EC10]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT171667 NZ_CP078013:c2500148-2499783 [Pseudomonas pergaminensis]
GTGAACAATCAACTGCAACACAAAGGCTACATCGGCTCTATCGAAGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAGAT
TCTGTTCATCAAGGCGCTGGTGAGCTACGAAGGAAAAACCGTCGCCGAGCTGGACGGCGCGTTCCGGGAGGCGGTGGACG
ACTACCTCGCCACCTGCCAGGCCCTGGGGCAAACACCGGAAAAACCCTGCAAGGGTTCATTCAACGTACGCGTCGGCCAT
GACTTGCACTTGGCGGCAGCCCTGGCGGCGACCCGCAAAAAAGTGACCCTCAATGACCTGACACGCCAGGCCTTGAATGA
GTTTTTACAACATCATGGCAACGACCCGTGCCCTGCGGTTAGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References