Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3495146..3495643 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9197_RS16800 Protein ID WP_001173343.1
Coordinates 3495470..3495643 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9197_RS16795 Protein ID WP_000398619.1
Coordinates 3495146..3495442 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS16775 (3491877) 3491877..3492596 - 720 WP_000611288.1 two-component system response regulator DcuR -
H9197_RS16780 (3492593) 3492593..3494224 - 1632 WP_001216460.1 sensor histidine kinase -
H9197_RS16785 (3494405) 3494405..3494635 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
H9197_RS16790 (3494647) 3494647..3494919 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
H9197_RS16795 (3495146) 3495146..3495442 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9197_RS16800 (3495470) 3495470..3495643 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9197_RS16805 (3495762) 3495762..3497279 - 1518 WP_023155344.1 lysine--tRNA ligase -
H9197_RS16810 (3497516) 3497516..3498973 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171666 WP_001173343.1 NZ_CP060946:3495470-3495643 [Escherichia coli O157:H7 str. EC10]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171666 NZ_CP078013:2020623-2020811 [Pseudomonas pergaminensis]
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCAGGTTGGGTGCTGGATCGGGTCACGGGAAGTCATCATATTTT
TACCCATCGCTATAGCCCGTACACCCTTCCAGTGCCCCATCCAAAGAAGGACTTGCCGCTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171666 WP_000398619.1 NZ_CP060946:3495146-3495442 [Escherichia coli O157:H7 str. EC10]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171666 NZ_CP078013:2020823-2021227 [Pseudomonas pergaminensis]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACTGCCACCGGTATCCAGATCCCCGATATCCCAGGTGCAGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTACAACGCCGCCGTGGAAGTCGCGCACATCATGCTGCAAGAGATCGCCGCAG
AGGGCGGGACGATTCCGATGCCCACGTCGGTCGCCCAGTACCATGCCCACGAGGACTACGCCGGGATGGGCTGGGGCATG
TTGGAGCTGGACGTCACGCCCTATCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCCGGTTATGTCATCCAGCGCAT
CGACCGCTATGTGCGAGAGCACAAGGTCAAAAGCCGCTCGTCATTCTTGGCGGACGCGGCATTGGAGAAGTTGGTGCGGT
CCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References