Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | V | Classification (family/domain) | ghoTS/ghoT-GhoS |
Location | 3495146..3495643 | Replicon | chromosome |
Accession | NZ_CP060946 | ||
Organism | Escherichia coli O157:H7 str. EC10 |
Toxin (Protein)
Gene name | ghoT | Uniprot ID | L4JJG6 |
Locus tag | H9197_RS16800 | Protein ID | WP_001173343.1 |
Coordinates | 3495470..3495643 (+) | Length | 58 a.a. |
Antitoxin (Protein)
Gene name | ghoS | Uniprot ID | L4JK92 |
Locus tag | H9197_RS16795 | Protein ID | WP_000398619.1 |
Coordinates | 3495146..3495442 (+) | Length | 99 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
H9197_RS16775 (3491877) | 3491877..3492596 | - | 720 | WP_000611288.1 | two-component system response regulator DcuR | - |
H9197_RS16780 (3492593) | 3492593..3494224 | - | 1632 | WP_001216460.1 | sensor histidine kinase | - |
H9197_RS16785 (3494405) | 3494405..3494635 | + | 231 | WP_000371698.1 | 4Fe-4S mono-cluster protein YjdI | - |
H9197_RS16790 (3494647) | 3494647..3494919 | + | 273 | WP_000405647.1 | GNAT family N-acetyltransferase | - |
H9197_RS16795 (3495146) | 3495146..3495442 | + | 297 | WP_000398619.1 | type V toxin-antitoxin system endoribonuclease antitoxin GhoS | Antitoxin |
H9197_RS16800 (3495470) | 3495470..3495643 | + | 174 | WP_001173343.1 | type V toxin-antitoxin system toxin GhoT | Toxin |
H9197_RS16805 (3495762) | 3495762..3497279 | - | 1518 | WP_023155344.1 | lysine--tRNA ligase | - |
H9197_RS16810 (3497516) | 3497516..3498973 | - | 1458 | WP_000856841.1 | dipeptide/tripeptide permease DtpC | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 58 a.a. Molecular weight: 6555.06 Da Isoelectric Point: 10.2288
>T171666 WP_001173343.1 NZ_CP060946:3495470-3495643 [Escherichia coli O157:H7 str. EC10]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF
Download Length: 174 bp
>T171666 NZ_CP078013:2020623-2020811 [Pseudomonas pergaminensis]
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCAGGTTGGGTGCTGGATCGGGTCACGGGAAGTCATCATATTTT
TACCCATCGCTATAGCCCGTACACCCTTCCAGTGCCCCATCCAAAGAAGGACTTGCCGCTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA
GTGCAAAGCAGGTTATTGATCAAGGAACTGCAAGAGGCAGGTTGGGTGCTGGATCGGGTCACGGGAAGTCATCATATTTT
TACCCATCGCTATAGCCCGTACACCCTTCCAGTGCCCCATCCAAAGAAGGACTTGCCGCTGGGCACCGTCAGAAGCATCA
GGAAACGCGCCGGCTTGTTTCAGTTTTAA
Antitoxin
Download Length: 99 a.a. Molecular weight: 11467.97 Da Isoelectric Point: 4.1705
>AT171666 WP_000398619.1 NZ_CP060946:3495146-3495442 [Escherichia coli O157:H7 str. EC10]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK
Download Length: 297 bp
>AT171666 NZ_CP078013:2020823-2021227 [Pseudomonas pergaminensis]
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACTGCCACCGGTATCCAGATCCCCGATATCCCAGGTGCAGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTACAACGCCGCCGTGGAAGTCGCGCACATCATGCTGCAAGAGATCGCCGCAG
AGGGCGGGACGATTCCGATGCCCACGTCGGTCGCCCAGTACCATGCCCACGAGGACTACGCCGGGATGGGCTGGGGCATG
TTGGAGCTGGACGTCACGCCCTATCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCCGGTTATGTCATCCAGCGCAT
CGACCGCTATGTGCGAGAGCACAAGGTCAAAAGCCGCTCGTCATTCTTGGCGGACGCGGCATTGGAGAAGTTGGTGCGGT
CCTAG
ATGCAATACCCAATCTGTATCGAATGGGGCGATGACTTCACTGCCACCGGTATCCAGATCCCCGATATCCCAGGTGCAGT
CACCGCCGGGGACAGTTTTGAAGAGGCGTACAACGCCGCCGTGGAAGTCGCGCACATCATGCTGCAAGAGATCGCCGCAG
AGGGCGGGACGATTCCGATGCCCACGTCGGTCGCCCAGTACCATGCCCACGAGGACTACGCCGGGATGGGCTGGGGCATG
TTGGAGCTGGACGTCACGCCCTATCTGGGCAAAACCGAGAAGGTCAATGTGACCCTGCCCGGTTATGTCATCCAGCGCAT
CGACCGCTATGTGCGAGAGCACAAGGTCAAAAGCCGCTCGTCATTCTTGGCGGACGCGGCATTGGAGAAGTTGGTGCGGT
CCTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7U9QI41 |
Antitoxin
Source | ID | Structure |
---|---|---|
PDB | 2LLZ | |
AlphaFold DB | A0A7U9QBH6 |