Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2741162..2741748 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag H9197_RS13325 Protein ID WP_042014162.1
Coordinates 2741518..2741748 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H9197_RS13320 Protein ID WP_000593555.1
Coordinates 2741162..2741521 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS13295 (2737294) 2737294..2738238 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
H9197_RS13300 (2738235) 2738235..2739068 + 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
H9197_RS13305 (2739068) 2739068..2739832 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H9197_RS13310 (2739829) 2739829..2740635 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H9197_RS13315 (2740641) 2740641..2741042 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H9197_RS13320 (2741162) 2741162..2741521 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H9197_RS13325 (2741518) 2741518..2741748 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
H9197_RS13330 (2741852) 2741852..2742976 - 1125 WP_241407715.1 ABC transporter permease -
H9197_RS13335 (2742976) 2742976..2745711 - 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T171664 WP_042014162.1 NZ_CP060946:c2741748-2741518 [Escherichia coli O157:H7 str. EC10]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T171664 NZ_CP078009:c5575922-5575644 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT171664 WP_000593555.1 NZ_CP060946:c2741521-2741162 [Escherichia coli O157:H7 str. EC10]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT171664 NZ_CP078009:c5575632-5575327 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References