Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2147342..2147996 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H9197_RS10325 Protein ID WP_000244772.1
Coordinates 2147342..2147749 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9197_RS10330 Protein ID WP_000354046.1
Coordinates 2147730..2147996 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS10305 (2143299) 2143299..2145032 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
H9197_RS10310 (2145038) 2145038..2145748 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9197_RS10315 (2145773) 2145773..2146669 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9197_RS10320 (2146781) 2146781..2147302 + 522 WP_001055874.1 flavodoxin FldB -
H9197_RS10325 (2147342) 2147342..2147749 - 408 WP_000244772.1 protein YgfX Toxin
H9197_RS10330 (2147730) 2147730..2147996 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9197_RS10335 (2148239) 2148239..2149219 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9197_RS10340 (2149296) 2149296..2149955 - 660 WP_000250274.1 hemolysin III family protein -
H9197_RS10345 (2150119) 2150119..2150430 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9197_RS10350 (2150475) 2150475..2151908 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H9197_RS10355 (2151965) 2151965..2152708 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T171662 WP_000244772.1 NZ_CP060946:c2147749-2147342 [Escherichia coli O157:H7 str. EC10]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171662 NZ_CP078009:c5320471-5320181 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171662 WP_000354046.1 NZ_CP060946:c2147996-2147730 [Escherichia coli O157:H7 str. EC10]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171662 NZ_CP078009:c5320767-5320468 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References