Detailed information of TA system TA171643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 26982..27507
• Replicon: plasmid pEC11-2
• Accession: NZ_CP060939
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: H9198_RS23335
• Protein ID: WP_013023785.1
• Coordinates: 27202..27507 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: H9198_RS23330
• Protein ID: WP_004197642.1
• Coordinates: 26982..27200 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9198_RS23310 (H9198_23095)	22482..22790	+	309	WP_001568014.1	hypothetical protein	-
H9198_RS24860	22890..23012	+	123	WP_256046161.1	hypothetical protein	-
H9198_RS23315 (H9198_23100)	23431..25569	+	2139	WP_048295729.1	AAA family ATPase	-
H9198_RS23320 (H9198_23105)	25739..26155	-	417	WP_080884179.1	type II toxin-antitoxin system VapC family toxin	-
H9198_RS23325 (H9198_23110)	26152..26382	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
H9198_RS23330 (H9198_23115)	26982..27200	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H9198_RS23335 (H9198_23120)	27202..27507	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
H9198_RS23340 (H9198_23125)	27676..28071	+	396	WP_072158608.1	hypothetical protein	-
H9198_RS23345 (H9198_23130)	28098..28421	+	324	WP_022644730.1	hypothetical protein	-
H9198_RS23350 (H9198_23135)	28418..29434	+	1017	WP_020315256.1	hypothetical protein	-
H9198_RS23355 (H9198_23140)	29769..31037	-	1269	WP_019705993.1	ISL3-like element ISKpn25 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..116611	116611
	-	flank	IS/Tn	-	-	29769..31037	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T171643 WP_013023785.1 NZ_CP060939:27202-27507 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T171643 NZ_CP078006:1427683-1428093 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT171643 WP_004197642.1 NZ_CP060939:26982-27200 [Escherichia coli]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT171643 NZ_CP078006:1427441-1427683 [Pseudomonas aeruginosa]
ATGAGGTGTGTCGTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCT
TCCTGATGACGTTAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATA
GCTGGTTCGATGGCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTC
TGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6