Detailed information of TA system TA171642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 25739..26382
• Replicon: plasmid pEC11-2
• Accession: NZ_CP060939
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: H9198_RS23320
• Protein ID: WP_080884179.1
• Coordinates: 25739..26155 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: H9198_RS23325
• Protein ID: WP_001261276.1
• Coordinates: 26152..26382 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9198_RS23300 (H9198_23085)	20959..21393	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
H9198_RS23305 (H9198_23090)	21440..21988	-	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
H9198_RS23310 (H9198_23095)	22482..22790	+	309	WP_001568014.1	hypothetical protein	-
H9198_RS24860	22890..23012	+	123	WP_256046161.1	hypothetical protein	-
H9198_RS23315 (H9198_23100)	23431..25569	+	2139	WP_048295729.1	AAA family ATPase	-
H9198_RS23320 (H9198_23105)	25739..26155	-	417	WP_080884179.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9198_RS23325 (H9198_23110)	26152..26382	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
H9198_RS23330 (H9198_23115)	26982..27200	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	-
H9198_RS23335 (H9198_23120)	27202..27507	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
H9198_RS23340 (H9198_23125)	27676..28071	+	396	WP_072158608.1	hypothetical protein	-
H9198_RS23345 (H9198_23130)	28098..28421	+	324	WP_022644730.1	hypothetical protein	-
H9198_RS23350 (H9198_23135)	28418..29434	+	1017	WP_020315256.1	hypothetical protein	-
H9198_RS23355 (H9198_23140)	29769..31037	-	1269	WP_019705993.1	ISL3-like element ISKpn25 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..116611	116611
	-	flank	IS/Tn	-	-	29769..31037	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.58 Da
• Isoelectric Point: 8.5403

>T171642 WP_080884179.1 NZ_CP060939:c26155-25739 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPALVLEDWVK

Nucleotide

• Length: 417 bp

>T171642 NZ_CP078006:c176383-176102 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT171642 WP_001261276.1 NZ_CP060939:c26382-26152 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT171642 NZ_CP078006:c176607-176380 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023