Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4181573..4182191 Replicon chromosome
Accession NZ_CP060938
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9198_RS20220 Protein ID WP_001291435.1
Coordinates 4181973..4182191 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9198_RS20215 Protein ID WP_000344800.1
Coordinates 4181573..4181947 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9198_RS20205 (4176662) 4176662..4177855 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9198_RS20210 (4177878) 4177878..4181027 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9198_RS20215 (4181573) 4181573..4181947 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9198_RS20220 (4181973) 4181973..4182191 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9198_RS20225 (4182363) 4182363..4182914 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9198_RS20230 (4183030) 4183030..4183500 + 471 WP_000136192.1 YlaC family protein -
H9198_RS20235 (4183664) 4183664..4185214 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9198_RS20240 (4185256) 4185256..4185609 - 354 WP_000878140.1 DUF1428 domain-containing protein -
H9198_RS20250 (4185988) 4185988..4186299 + 312 WP_000409911.1 MGMT family protein -
H9198_RS20255 (4186330) 4186330..4186902 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171638 WP_001291435.1 NZ_CP060938:4181973-4182191 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171638 NZ_CP078004:c4794295-4793885 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171638 WP_000344800.1 NZ_CP060938:4181573-4181947 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171638 NZ_CP078004:c4794525-4794295 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References