Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4446827..4447403 Replicon chromosome
Accession NZ_CP060933
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag H9199_RS21905 Protein ID WP_001131965.1
Coordinates 4447116..4447403 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag H9199_RS21900 Protein ID WP_019842999.1
Coordinates 4446827..4447129 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9199_RS21880 (4442440) 4442440..4442952 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
H9199_RS21885 (4443342) 4443342..4445492 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
H9199_RS21890 (4445623) 4445623..4445826 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9199_RS21895 (4445837) 4445837..4446793 + 957 WP_001295525.1 GTPase -
H9199_RS21900 (4446827) 4446827..4447129 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
H9199_RS21905 (4447116) 4447116..4447403 - 288 WP_001131965.1 BrnT family toxin Toxin
H9199_RS21910 (4447600) 4447600..4448766 + 1167 WP_000800825.1 restriction endonuclease -
H9199_RS21915 (4448830) 4448830..4450449 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -
H9199_RS21920 (4450439) 4450439..4451740 + 1302 WP_100729279.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaCTX-M-55 cheD / fimH / fimG / fimF / fimD / fimC / fimI / fimA / fimE / fimB 4364165..4534448 170283
- flank IS/Tn - - 4451734..4452111 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T171612 WP_001131965.1 NZ_CP060933:c4447403-4447116 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171612 NZ_CP077997:2501149-2501430 [Pseudomonas aeruginosa]
ATGACCTATAAGCTCGAATTCCTGCCCTCGGCGCTGAAGGAGTGGGAGAAGCTGGGTCATACGCTGCGTGAACAGGCAAA
GAAAAAGCTTCGCGAGCGATTGGAAGCACCAAAGGTACAGACTGATGCCCTCAGGGATCTGCCTGGGCACTACAAAATCA
AGCTCCGAACAGCAGGCTATCGATTGGTCTACCGGGTCGAAGATGATCGAGTGGTTGTAGTGGTGGTCGCAGTTGGCAAA
CGTGAGCGAGGTGCTGCTTACAAGGCAGCAGCTAAGCGTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT171612 WP_019842999.1 NZ_CP060933:c4447129-4446827 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171612 NZ_CP077997:2500914-2501159 [Pseudomonas aeruginosa]
ATGCAGAGTGTTTTGGCCGACGTTGCCGTGAGCGTTTCTGAGCTGAAGAAAAACCCCACTGGAGTCATGGCTGGTGCCGG
AGGCATGCCGGTCGCCGTTCTTAATCATAACCGCGTGATGGGGTACATGGTGCCTGCCGAGCTGTACGAGCAGATGCTGG
ACCGTCTGGACGATCTGGATCTAATCGATGTCGTTAACGCACGTTCCGGTGAGAAGGGTGTGCCGGTGAACCTGGATGAC
CTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References