Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2781544..2782182 Replicon chromosome
Accession NZ_CP060933
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H9199_RS13735 Protein ID WP_000813794.1
Coordinates 2782006..2782182 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H9199_RS13730 Protein ID WP_001270286.1
Coordinates 2781544..2781960 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9199_RS13710 (2776696) 2776696..2777637 - 942 WP_072649490.1 ABC transporter permease -
H9199_RS13715 (2777638) 2777638..2778651 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
H9199_RS13720 (2778669) 2778669..2779814 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
H9199_RS13725 (2780059) 2780059..2781465 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
H9199_RS13730 (2781544) 2781544..2781960 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H9199_RS13735 (2782006) 2782006..2782182 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H9199_RS13740 (2782404) 2782404..2782634 + 231 WP_000494244.1 YncJ family protein -
H9199_RS13745 (2782726) 2782726..2784687 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H9199_RS13750 (2784760) 2784760..2785296 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H9199_RS13755 (2785388) 2785388..2786563 + 1176 WP_001236215.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T171608 WP_000813794.1 NZ_CP060933:c2782182-2782006 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T171608 NZ_CP077996:c9734-9459 [Pseudomonas aeruginosa]
ATGGAGTTGAAGTGGACGAGCAAAGGGCTCTCCGACGTGGCACGGCTCTACGAGTTCCTGGCTACGGTGAACAGGGAAGC
TGCCGCCCGCACGGTGCAGCAGCTGACGAGCGCGCCGACCGCCTTGTTGACCAACCCGCGGCTGGGCGAGCGCCTGGAAG
AGTTCGAACCGCGGGATGTTCGCCGCATCCTGATCGGCCACTATGAGATGCGCTATGAGATCCTGGATTCCTGCATCTAC
GTGCTTCGTCTGTGGCACACACGCGAAGACAGGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT171608 WP_001270286.1 NZ_CP060933:c2781960-2781544 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT171608 NZ_CP077996:c10009-9734 [Pseudomonas aeruginosa]
ATGAGCACAGCAGCCAAACCAACGCGTACCGTGACTGCTCACGTCCCGGTAGATCTGGCCGAGCGCGTCGATGCAATCGC
CGAGCGCCTGGAGCGATCGAAGAACTGGATCGTCAGGCAGGCGCTGTCAGCCTGGATCGACCAGGAAGACGAGCGTAGCC
GTCTGACACGTGAAGCCTTGGATGATGTAGACGCAGGACGTGTCGTCGACCACCAGGCCGTCCAGGCCTGGGCCGAAAGC
CTGGGCACTGACTCTCCGCTGCCGGTACCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References