Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1639596..1640221 Replicon chromosome
Accession NZ_CP060933
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag H9199_RS08160 Protein ID WP_000911330.1
Coordinates 1639823..1640221 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag H9199_RS08155 Protein ID WP_000450524.1
Coordinates 1639596..1639823 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9199_RS08130 (1635399) 1635399..1635869 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
H9199_RS08135 (1635869) 1635869..1636441 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
H9199_RS08140 (1636587) 1636587..1637465 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
H9199_RS08145 (1637482) 1637482..1638516 + 1035 WP_001330579.1 outer membrane protein assembly factor BamC -
H9199_RS08150 (1638729) 1638729..1639442 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
H9199_RS08155 (1639596) 1639596..1639823 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
H9199_RS08160 (1639823) 1639823..1640221 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
H9199_RS08165 (1640368) 1640368..1641231 + 864 WP_001267498.1 neutral zinc metallopeptidase -
H9199_RS08170 (1641246) 1641246..1643261 + 2016 WP_000829292.1 tRNA cytosine(34) acetyltransferase TmcA -
H9199_RS08175 (1643335) 1643335..1644033 + 699 WP_000679812.1 esterase -
H9199_RS08180 (1644114) 1644114..1644314 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T171597 WP_000911330.1 NZ_CP060933:1639823-1640221 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T171597 NZ_CP077988:c5532752-5532474 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT171597 WP_000450524.1 NZ_CP060933:1639596-1639823 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT171597 NZ_CP077988:c5532462-5532157 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References