Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1187417..1188071 Replicon chromosome
Accession NZ_CP060933
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H9199_RS05925 Protein ID WP_000244772.1
Coordinates 1187664..1188071 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9199_RS05920 Protein ID WP_000354046.1
Coordinates 1187417..1187683 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9199_RS05895 (1182705) 1182705..1183448 + 744 WP_000951964.1 SDR family oxidoreductase -
H9199_RS05900 (1183505) 1183505..1184938 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
H9199_RS05905 (1184983) 1184983..1185294 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9199_RS05910 (1185458) 1185458..1186117 + 660 WP_000250274.1 hemolysin III family protein -
H9199_RS05915 (1186194) 1186194..1187174 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9199_RS05920 (1187417) 1187417..1187683 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9199_RS05925 (1187664) 1187664..1188071 + 408 WP_000244772.1 protein YgfX Toxin
H9199_RS05930 (1188111) 1188111..1188632 - 522 WP_001055874.1 flavodoxin FldB -
H9199_RS05935 (1188744) 1188744..1189640 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9199_RS05940 (1189665) 1189665..1190375 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9199_RS05945 (1190381) 1190381..1192114 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T171595 WP_000244772.1 NZ_CP060933:1187664-1188071 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171595 NZ_CP077988:c4604437-4603682 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171595 WP_000354046.1 NZ_CP060933:1187417-1187683 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171595 NZ_CP077988:c4604940-4604572 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTGGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References