Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 511957..512588 Replicon chromosome
Accession NZ_CP060933
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H9199_RS02450 Protein ID WP_001259388.1
Coordinates 511957..512232 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H9199_RS02455 Protein ID WP_000593555.1
Coordinates 512229..512588 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9199_RS02435 (506961) 506961..508028 + 1068 WP_000361485.1 HlyD family secretion protein -
H9199_RS02440 (508025) 508025..510760 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
H9199_RS02445 (510760) 510760..511884 + 1125 WP_001314210.1 ABC transporter permease -
H9199_RS02450 (511957) 511957..512232 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H9199_RS02455 (512229) 512229..512588 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H9199_RS02460 (512708) 512708..513109 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H9199_RS02465 (513115) 513115..513921 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H9199_RS02470 (513918) 513918..514682 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H9199_RS02475 (514682) 514682..515515 - 834 WP_038999760.1 nickel ABC transporter permease subunit NikC -
H9199_RS02480 (515512) 515512..516456 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T171591 WP_001259388.1 NZ_CP060933:511957-512232 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T171591 NZ_CP077988:c143521-143240 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT171591 WP_000593555.1 NZ_CP060933:512229-512588 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT171591 NZ_CP077988:c143745-143518 [Pseudomonas aeruginosa]
ATGAGCACTGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References