Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 62168..62769 Replicon plasmid pEC13-3
Accession NZ_CP060930
Organism Escherichia coli strain EC13

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag H9200_RS24080 Protein ID WP_001216045.1
Coordinates 62168..62548 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H9200_RS24085 Protein ID WP_001190712.1
Coordinates 62548..62769 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9200_RS24815 57295..57525 - 231 Protein_58 ash family protein -
H9200_RS24055 (H9200_23830) 57609..59051 - 1443 WP_224114847.1 terminase -
H9200_RS24060 (H9200_23835) 59093..60286 - 1194 WP_241424601.1 terminase -
H9200_RS24065 (H9200_23840) 60372..60824 - 453 WP_032227319.1 Late promoter-activating protein -
H9200_RS24070 (H9200_23845) 60913..61956 - 1044 WP_000648823.1 DUF968 domain-containing protein -
H9200_RS24075 (H9200_23850) 61984..62163 - 180 WP_001339207.1 hypothetical protein -
H9200_RS24080 (H9200_23855) 62168..62548 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H9200_RS24085 (H9200_23860) 62548..62769 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H9200_RS24090 (H9200_23865) 62842..63231 - 390 WP_000506724.1 S24 family peptidase -
H9200_RS24095 (H9200_23870) 63355..63606 - 252 WP_001377386.1 DNA polymerase III subunit theta -
H9200_RS24100 (H9200_23875) 64056..64193 + 138 WP_000123562.1 hypothetical protein -
H9200_RS24105 (H9200_23880) 64268..64630 - 363 WP_001261544.1 hypothetical protein -
H9200_RS24110 (H9200_23885) 64627..65271 - 645 WP_104769659.1 hypothetical protein -
H9200_RS24115 (H9200_23890) 65253..65627 - 375 WP_033553822.1 hypothetical protein -
H9200_RS24120 (H9200_23895) 65634..65927 - 294 WP_000268000.1 hypothetical protein -
H9200_RS24125 (H9200_23900) 66106..66339 - 234 WP_000517420.1 hypothetical protein -
H9200_RS24130 (H9200_23905) 66416..66676 - 261 WP_033553819.1 hypothetical protein -
H9200_RS24820 66673..66990 - 318 WP_259620635.1 DUF551 domain-containing protein -
H9200_RS24140 67258..67518 - 261 Protein_76 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94661 94661


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T171589 WP_001216045.1 NZ_CP060930:c62548-62168 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T171589 NZ_CP077985:c5608779-5608501 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT171589 WP_001190712.1 NZ_CP060930:c62769-62548 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT171589 NZ_CP077985:c5608489-5608184 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References