Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4210243..4210861 Replicon chromosome
Accession NZ_CP060928
Organism Escherichia coli strain EC13

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9200_RS20465 Protein ID WP_001291435.1
Coordinates 4210243..4210461 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9200_RS20470 Protein ID WP_000344800.1
Coordinates 4210487..4210861 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9200_RS20430 (4205532) 4205532..4206104 + 573 WP_000779831.1 YbaY family lipoprotein -
H9200_RS20435 (4206135) 4206135..4206446 - 312 WP_000409911.1 MGMT family protein -
H9200_RS20445 (4206825) 4206825..4207178 + 354 WP_000878140.1 DUF1428 domain-containing protein -
H9200_RS20450 (4207220) 4207220..4208770 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9200_RS20455 (4208934) 4208934..4209404 - 471 WP_000136192.1 YlaC family protein -
H9200_RS20460 (4209520) 4209520..4210071 - 552 WP_000102564.1 maltose O-acetyltransferase -
H9200_RS20465 (4210243) 4210243..4210461 - 219 WP_001291435.1 HHA domain-containing protein Toxin
H9200_RS20470 (4210487) 4210487..4210861 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9200_RS20475 (4211407) 4211407..4214556 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9200_RS20480 (4214579) 4214579..4215772 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171580 WP_001291435.1 NZ_CP060928:c4210461-4210243 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171580 NZ_CP060928:c4210461-4210243 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171580 WP_000344800.1 NZ_CP060928:c4210861-4210487 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171580 NZ_CP060928:c4210861-4210487 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References