Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3649235..3649811 Replicon chromosome
Accession NZ_CP060928
Organism Escherichia coli strain EC13

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag H9200_RS17755 Protein ID WP_001297643.1
Coordinates 3649235..3649522 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag H9200_RS17760 Protein ID WP_000063156.1
Coordinates 3649509..3649811 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9200_RS17735 (3645488) 3645488..3646768 - 1281 WP_001037380.1 DUF445 domain-containing protein -
H9200_RS17740 (3646809) 3646809..3648041 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
H9200_RS17745 (3648187) 3648187..3648282 - 96 Protein_3469 dienelactone hydrolase family protein -
H9200_RS17750 (3648339) 3648339..3649036 + 698 Protein_3470 IS1 family transposase -
H9200_RS17755 (3649235) 3649235..3649522 + 288 WP_001297643.1 BrnT family toxin Toxin
H9200_RS17760 (3649509) 3649509..3649811 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
H9200_RS17765 (3649845) 3649845..3650801 - 957 WP_001297640.1 GTPase -
H9200_RS17770 (3650812) 3650812..3651015 - 204 WP_000467859.1 YbdD/YjiX family protein -
H9200_RS17775 (3651065) 3651065..3653215 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 3648533..3717227 68694


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T171577 WP_001297643.1 NZ_CP060928:3649235-3649522 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171577 NZ_CP077981:c144365-144084 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT171577 WP_000063156.1 NZ_CP060928:3649509-3649811 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171577 NZ_CP077981:c144589-144362 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATAGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References