Detailed information of TA system TA171558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 561314..561952
• Replicon: chromosome
• Accession: NZ_CP060928
• Organism: Escherichia coli strain EC13

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H9200_RS02810
• Protein ID: WP_001447010.1
• Coordinates: 561314..561490 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H9200_RS02815
• Protein ID: WP_001270286.1
• Coordinates: 561536..561952 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9200_RS02790 (556933)	556933..558108	-	1176	WP_001236265.1	BenE family transporter YdcO	-
H9200_RS02795 (558200)	558200..558736	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H9200_RS02800 (558809)	558809..560770	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H9200_RS02805 (560862)	560862..561092	-	231	WP_000494244.1	YncJ family protein	-
H9200_RS02810 (561314)	561314..561490	+	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H9200_RS02815 (561536)	561536..561952	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H9200_RS02820 (562031)	562031..563437	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H9200_RS02825 (563682)	563682..564827	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H9200_RS02830 (564845)	564845..565858	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H9200_RS02835 (565859)	565859..566800	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T171558 WP_001447010.1 NZ_CP060928:561314-561490 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T171558 NZ_CP077969:4266724-4266897 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT171558 WP_001270286.1 NZ_CP060928:561536-561952 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT171558 NZ_CP077969:4266400-4266696 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8