Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4350199..4350696 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9201_RS21135 Protein ID WP_001173343.1
Coordinates 4350199..4350372 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9201_RS21140 Protein ID WP_000398619.1
Coordinates 4350400..4350696 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS21125 (4346869) 4346869..4348326 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9201_RS21130 (4348563) 4348563..4350080 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9201_RS21135 (4350199) 4350199..4350372 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9201_RS21140 (4350400) 4350400..4350696 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9201_RS21145 (4350923) 4350923..4351195 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
H9201_RS21150 (4351207) 4351207..4351437 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
H9201_RS21155 (4351618) 4351618..4353249 + 1632 WP_001216487.1 sensor histidine kinase -
H9201_RS21160 (4353246) 4353246..4353965 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171546 WP_001173343.1 NZ_CP060924:c4350372-4350199 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171546 NZ_CP077969:c1564446-1564159 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171546 WP_000398619.1 NZ_CP060924:c4350696-4350400 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171546 NZ_CP077969:c1564685-1564446 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References