Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4087576..4088152 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag H9201_RS19815 Protein ID WP_001131965.1
Coordinates 4087865..4088152 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag H9201_RS19810 Protein ID WP_019842999.1
Coordinates 4087576..4087878 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS19790 (4083189) 4083189..4083701 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
H9201_RS19795 (4084091) 4084091..4086241 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
H9201_RS19800 (4086372) 4086372..4086575 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9201_RS19805 (4086586) 4086586..4087542 + 957 WP_001295525.1 GTPase -
H9201_RS19810 (4087576) 4087576..4087878 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
H9201_RS19815 (4087865) 4087865..4088152 - 288 WP_001131965.1 BrnT family toxin Toxin
H9201_RS19820 (4088349) 4088349..4089515 + 1167 WP_000800825.1 restriction endonuclease -
H9201_RS19825 (4089579) 4089579..4091198 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -
H9201_RS19830 (4091188) 4091188..4092612 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T171543 WP_001131965.1 NZ_CP060924:c4088152-4087865 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171543 NZ_CP077969:c1331405-1331211 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT171543 WP_019842999.1 NZ_CP060924:c4087878-4087576 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171543 NZ_CP077969:1331034-1331212 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References