171541

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3513576..3514413 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag H9201_RS16980 Protein ID WP_000227784.1
Coordinates 3513871..3514413 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag H9201_RS16975 Protein ID WP_001297137.1
Coordinates 3513576..3513887 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS16950 (3508596) 3508596..3509543 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
H9201_RS16955 (3509565) 3509565..3511556 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H9201_RS16960 (3511546) 3511546..3512160 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H9201_RS16965 (3512160) 3512160..3512489 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
H9201_RS16970 (3512501) 3512501..3513391 + 891 WP_000971336.1 heme o synthase -
H9201_RS16975 (3513576) 3513576..3513887 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
H9201_RS16980 (3513871) 3513871..3514413 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
H9201_RS16985 (3514469) 3514469..3515404 - 936 WP_001365761.1 tetratricopeptide repeat protein -
H9201_RS16990 (3515812) 3515812..3517176 + 1365 WP_001000974.1 MFS transporter -
H9201_RS16995 (3517304) 3517304..3517795 - 492 WP_001138904.1 nucleotide binding protein YajQ -
H9201_RS17000 (3517963) 3517963..3518874 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T171541 WP_000227784.1 NZ_CP060924:3513871-3514413 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T171541 NZ_CP077969:c1139332-1139225 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT171541 WP_001297137.1 NZ_CP060924:3513576-3513887 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT171541 NZ_CP077969:1139379-1139445 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References