Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3479500..3480118 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9201_RS16810 Protein ID WP_001291435.1
Coordinates 3479900..3480118 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9201_RS16805 Protein ID WP_000344800.1
Coordinates 3479500..3479874 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS16795 (3474589) 3474589..3475782 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9201_RS16800 (3475805) 3475805..3478954 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9201_RS16805 (3479500) 3479500..3479874 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9201_RS16810 (3479900) 3479900..3480118 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9201_RS16815 (3480289) 3480289..3480840 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9201_RS16820 (3480956) 3480956..3481426 + 471 WP_000136192.1 YlaC family protein -
H9201_RS16825 (3481590) 3481590..3483140 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9201_RS16830 (3483182) 3483182..3483535 - 354 WP_000878141.1 DUF1428 family protein -
H9201_RS16840 (3483914) 3483914..3484225 + 312 WP_000409911.1 MGMT family protein -
H9201_RS16845 (3484256) 3484256..3484828 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171540 WP_001291435.1 NZ_CP060924:3479900-3480118 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171540 NZ_CP077969:c1138797-1138690 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171540 WP_000344800.1 NZ_CP060924:3479500-3479874 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171540 NZ_CP077969:1138845-1138911 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References