Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2472114..2472752 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H9201_RS11935 Protein ID WP_000813794.1
Coordinates 2472576..2472752 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H9201_RS11930 Protein ID WP_001270286.1
Coordinates 2472114..2472530 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS11910 (2467266) 2467266..2468207 - 942 WP_001251315.1 ABC transporter permease -
H9201_RS11915 (2468208) 2468208..2469221 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
H9201_RS11920 (2469239) 2469239..2470384 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
H9201_RS11925 (2470629) 2470629..2472035 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
H9201_RS11930 (2472114) 2472114..2472530 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H9201_RS11935 (2472576) 2472576..2472752 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H9201_RS11940 (2472974) 2472974..2473204 + 231 WP_000494244.1 YncJ family protein -
H9201_RS11945 (2473296) 2473296..2475257 - 1962 WP_241415827.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H9201_RS11950 (2475330) 2475330..2475866 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
H9201_RS11955 (2475958) 2475958..2477133 + 1176 WP_001236215.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T171539 WP_000813794.1 NZ_CP060924:c2472752-2472576 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T171539 NZ_CP077969:c365034-364816 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT171539 WP_001270286.1 NZ_CP060924:c2472530-2472114 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT171539 NZ_CP077969:c365434-365060 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References