Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 943582..944236 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H9201_RS04640 Protein ID WP_000244772.1
Coordinates 943829..944236 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9201_RS04635 Protein ID WP_000354046.1
Coordinates 943582..943848 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS04610 (938870) 938870..939613 + 744 WP_000951964.1 SDR family oxidoreductase -
H9201_RS04615 (939670) 939670..941103 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
H9201_RS04620 (941148) 941148..941459 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9201_RS04625 (941623) 941623..942282 + 660 WP_000250274.1 hemolysin III family protein -
H9201_RS04630 (942359) 942359..943339 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9201_RS04635 (943582) 943582..943848 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9201_RS04640 (943829) 943829..944236 + 408 WP_000244772.1 protein YgfX Toxin
H9201_RS04645 (944276) 944276..944797 - 522 WP_001055874.1 flavodoxin FldB -
H9201_RS04650 (944909) 944909..945805 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9201_RS04655 (945830) 945830..946540 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9201_RS04660 (946546) 946546..948279 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T171526 WP_000244772.1 NZ_CP060924:943829-944236 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171526 NZ_CP077961:c9679-9329 [Mammaliicoccus sciuri]
ATGAATAGTATTTCACAAGGTACATTAATATATATAGATTTTGAACCATCAAAAGGTAGTGAAATAAAAAAAAGAAGACC
AGCTATTGTAATCAGTCGTGACGAATATAATTTAGCTTCCAATTTAGTGATCGTTTGTCCGATAACATCGACAGATAAAG
ATAGACCTTATTTTGTTCCAATTAATAATAAAAATTTAAAATCGAATAGTAAAGTAAATACTAAACAAGTATACAGTTTG
GATTATACAGAAAGAGCAGGAAGAAACATTCAAATAATCGGTAAGATTACCAATAAAGAATTAATGAATATAGCTCAGCA
TTTCCTAATGAATTTTAACTTTAATTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171526 WP_000354046.1 NZ_CP060924:943582-943848 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171526 NZ_CP077961:c9905-9672 [Mammaliicoccus sciuri]
ATGGATAAAGTAAAAACAAGAAAACAAGGTAATGCTGTGATGGTAACAATAGCCAAAAAATTCAATGTTCCTGAAGGACA
AGAATTCTATATAACAAAAGAATCAGACGGAACAATTTCTCTTATTCCTAAGATACAAGATTATTACAAGGAAGTAACGG
CAGGAGAATTTATAGATGAAGAAGATTCATTAGCTCAAAATTTTACAACTGCAGGAAATGAATTAGATGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References