Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 309046..309677 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H9201_RS01400 Protein ID WP_001259388.1
Coordinates 309046..309321 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H9201_RS01405 Protein ID WP_000593555.1
Coordinates 309318..309677 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS01385 (304050) 304050..305117 + 1068 WP_000361485.1 HlyD family secretion protein -
H9201_RS01390 (305114) 305114..307849 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
H9201_RS01395 (307849) 307849..308973 + 1125 WP_001314210.1 ABC transporter permease -
H9201_RS01400 (309046) 309046..309321 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H9201_RS01405 (309318) 309318..309677 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H9201_RS01410 (309797) 309797..310198 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H9201_RS01415 (310204) 310204..311010 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H9201_RS01420 (311007) 311007..311771 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H9201_RS01425 (311771) 311771..312604 - 834 WP_038999760.1 nickel ABC transporter permease subunit NikC -
H9201_RS01430 (312601) 312601..313545 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 292681..309677 16996


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T171523 WP_001259388.1 NZ_CP060924:309046-309321 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T171523 NZ_CP077945:612-956 [Leptospira interrogans serovar Bataviae]
TTGGTAAAGAATAGAATCTATACTCCTGAAAAAGGAGATATAGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTAACCTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT171523 WP_000593555.1 NZ_CP060924:309318-309677 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT171523 NZ_CP077945:382-618 [Leptospira interrogans serovar Bataviae]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References