Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 64222..64823 Replicon plasmid pEC15-3
Accession NZ_CP060921
Organism Escherichia coli strain EC15

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag H9202_RS25395 Protein ID WP_001216034.1
Coordinates 64222..64602 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H9202_RS25400 Protein ID WP_001190712.1
Coordinates 64602..64823 (-) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9202_RS25365 59321..59440 - 120 WP_071527722.1 ash family protein -
H9202_RS25370 (H9202_25105) 59663..61147 - 1485 WP_042016904.1 terminase -
H9202_RS25375 (H9202_25110) 61147..62340 - 1194 WP_029487616.1 hypothetical protein -
H9202_RS25380 (H9202_25115) 62426..62878 - 453 WP_001326849.1 late promoter-activating protein -
H9202_RS25385 (H9202_25120) 62967..64010 - 1044 WP_063090816.1 DUF968 domain-containing protein -
H9202_RS25390 (H9202_25125) 64038..64217 - 180 WP_000113018.1 hypothetical protein -
H9202_RS25395 (H9202_25130) 64222..64602 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H9202_RS25400 (H9202_25135) 64602..64823 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H9202_RS25405 (H9202_25140) 64896..65285 - 390 WP_000506730.1 S24 family peptidase -
H9202_RS25410 (H9202_25145) 66854..67216 - 363 WP_001261543.1 hypothetical protein -
H9202_RS25415 (H9202_25150) 67213..67560 - 348 WP_228252666.1 hypothetical protein -
H9202_RS25420 (H9202_25155) 67738..68244 - 507 WP_024245587.1 hypothetical protein -
H9202_RS25425 (H9202_25160) 68317..69579 - 1263 WP_024245586.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..91360 91360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T171522 WP_001216034.1 NZ_CP060921:c64602-64222 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T171522 NZ_CP077942:3299111-3299509 [Leptospira interrogans serovar Bataviae]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT171522 WP_001190712.1 NZ_CP060921:c64823-64602 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT171522 NZ_CP077942:3298881-3299111 [Leptospira interrogans serovar Bataviae]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTTCGACTACCAAAGGAATTTCAATTTGCCGGAGATGA
TGTTCTTATCCAAAAAGTTGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0AJ64


Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References