Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4421864..4422440 Replicon chromosome
Accession NZ_CP060919
Organism Escherichia coli strain EC15

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag H9202_RS21730 Protein ID WP_001131965.1
Coordinates 4422153..4422440 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag H9202_RS21725 Protein ID WP_019842999.1
Coordinates 4421864..4422166 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9202_RS21705 (4417477) 4417477..4417989 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
H9202_RS21710 (4418379) 4418379..4420529 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
H9202_RS21715 (4420660) 4420660..4420863 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9202_RS21720 (4420874) 4420874..4421830 + 957 WP_001295525.1 GTPase -
H9202_RS21725 (4421864) 4421864..4422166 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
H9202_RS21730 (4422153) 4422153..4422440 - 288 WP_001131965.1 BrnT family toxin Toxin
H9202_RS21735 (4422637) 4422637..4423803 + 1167 WP_000800825.1 restriction endonuclease -
H9202_RS21740 (4423867) 4423867..4425486 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaCTX-M-55 cheD / fimH / fimG / fimF / fimD / fimC / fimI / fimA / fimE / fimB 4339202..4508319 169117
- flank IS/Tn - - 4425605..4425982 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T171515 WP_001131965.1 NZ_CP060919:c4422440-4422153 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171515 NZ_CP077936:c2562332-2562066 [Staphylococcus aureus]
ATGGTTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTAATTAAAAGTATTGACAGAAATGGTGCGTTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT171515 WP_019842999.1 NZ_CP060919:c4422166-4421864 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171515 NZ_CP077936:c2562583-2562332 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATAGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTGTAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References