Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3805387..3806224 Replicon chromosome
Accession NZ_CP060919
Organism Escherichia coli strain EC15

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag H9202_RS18695 Protein ID WP_000227784.1
Coordinates 3805682..3806224 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag H9202_RS18690 Protein ID WP_001297137.1
Coordinates 3805387..3805698 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9202_RS18665 (3800407) 3800407..3801354 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
H9202_RS18670 (3801376) 3801376..3803367 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H9202_RS18675 (3803357) 3803357..3803971 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H9202_RS18680 (3803971) 3803971..3804300 + 330 WP_072649523.1 cytochrome o ubiquinol oxidase subunit IV -
H9202_RS18685 (3804312) 3804312..3805202 + 891 WP_000971336.1 heme o synthase -
H9202_RS18690 (3805387) 3805387..3805698 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
H9202_RS18695 (3805682) 3805682..3806224 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
H9202_RS18700 (3806280) 3806280..3807215 - 936 WP_001365761.1 tetratricopeptide repeat protein -
H9202_RS18705 (3807623) 3807623..3808987 + 1365 WP_001000974.1 MFS transporter -
H9202_RS18710 (3809115) 3809115..3809606 - 492 WP_001138904.1 nucleotide binding protein YajQ -
H9202_RS18715 (3809774) 3809774..3810685 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T171513 WP_000227784.1 NZ_CP060919:3805682-3806224 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T171513 NZ_CP077936:c2239625-2239416 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT171513 WP_001297137.1 NZ_CP060919:3805387-3805698 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT171513 NZ_CP077936:c2239930-2239637 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References