Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 63925..64526 Replicon plasmid pEC18-3
Accession NZ_CP060916
Organism Escherichia coli strain EC18

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag H9203_RS25395 Protein ID WP_001216034.1
Coordinates 63925..64305 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H9203_RS25400 Protein ID WP_001190712.1
Coordinates 64305..64526 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9203_RS25365 59024..59143 - 120 WP_071527722.1 ash family protein -
H9203_RS25370 (H9203_25105) 59366..60850 - 1485 WP_042016904.1 terminase -
H9203_RS25375 (H9203_25110) 60850..62043 - 1194 WP_029487616.1 hypothetical protein -
H9203_RS25380 (H9203_25115) 62129..62581 - 453 WP_001326849.1 late promoter-activating protein -
H9203_RS25385 (H9203_25120) 62670..63713 - 1044 WP_063090816.1 DUF968 domain-containing protein -
H9203_RS25390 (H9203_25125) 63741..63920 - 180 WP_000113018.1 hypothetical protein -
H9203_RS25395 (H9203_25130) 63925..64305 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H9203_RS25400 (H9203_25135) 64305..64526 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H9203_RS25405 (H9203_25140) 64599..64988 - 390 WP_000506730.1 S24 family peptidase -
H9203_RS25410 (H9203_25145) 66557..66919 - 363 WP_001261543.1 hypothetical protein -
H9203_RS25415 (H9203_25150) 66916..67263 - 348 WP_228252666.1 hypothetical protein -
H9203_RS25420 (H9203_25155) 67441..67947 - 507 WP_024245587.1 hypothetical protein -
H9203_RS25425 (H9203_25160) 68020..69282 - 1263 WP_024245586.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..91063 91063


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T171492 WP_001216034.1 NZ_CP060916:c64305-63925 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T171492 NZ_CP077932:c354500-354312 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT171492 WP_001190712.1 NZ_CP060916:c64526-64305 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT171492 NZ_CP077932:c354789-354529 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTACCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References