Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4685238..4685735 Replicon chromosome
Accession NZ_CP060914
Organism Escherichia coli strain EC18

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9203_RS23085 Protein ID WP_001173343.1
Coordinates 4685238..4685411 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9203_RS23090 Protein ID WP_000398619.1
Coordinates 4685439..4685735 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9203_RS23075 (4681908) 4681908..4683365 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9203_RS23080 (4683602) 4683602..4685119 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9203_RS23085 (4685238) 4685238..4685411 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9203_RS23090 (4685439) 4685439..4685735 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9203_RS23095 (4685962) 4685962..4686234 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
H9203_RS23100 (4686246) 4686246..4686476 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
H9203_RS23105 (4686657) 4686657..4688288 + 1632 WP_001216487.1 sensor histidine kinase -
H9203_RS23110 (4688285) 4688285..4689004 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171488 WP_001173343.1 NZ_CP060914:c4685411-4685238 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171488 NZ_CP077931:2554279-2554455 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171488 WP_000398619.1 NZ_CP060914:c4685735-4685439 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171488 NZ_CP077931:c2554617-2554443 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References