Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4503034..4503869 | Replicon | chromosome |
| Accession | NZ_CP060914 | ||
| Organism | Escherichia coli strain EC18 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A0J2AEA6 |
| Locus tag | H9203_RS22120 | Protein ID | WP_000854759.1 |
| Coordinates | 4503034..4503411 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | S1NM52 |
| Locus tag | H9203_RS22125 | Protein ID | WP_001295723.1 |
| Coordinates | 4503501..4503869 (-) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H9203_RS22095 (4499423) | 4499423..4500964 | - | 1542 | WP_001298859.1 | IS21-like element ISEc12 family transposase | - |
| H9203_RS22100 (4501558) | 4501558..4501734 | - | 177 | Protein_4321 | helix-turn-helix domain-containing protein | - |
| H9203_RS22105 (4502101) | 4502101..4502250 | - | 150 | Protein_4322 | hypothetical protein | - |
| H9203_RS22110 (4502356) | 4502356..4502532 | - | 177 | WP_000839286.1 | DUF957 domain-containing protein | - |
| H9203_RS22115 (4502549) | 4502549..4503037 | - | 489 | WP_000761690.1 | DUF5983 family protein | - |
| H9203_RS22120 (4503034) | 4503034..4503411 | - | 378 | WP_000854759.1 | TA system toxin CbtA family protein | Toxin |
| H9203_RS22125 (4503501) | 4503501..4503869 | - | 369 | WP_001295723.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| H9203_RS22130 (4504032) | 4504032..4504253 | - | 222 | WP_000692345.1 | DUF987 domain-containing protein | - |
| H9203_RS22135 (4504316) | 4504316..4504792 | - | 477 | WP_001186775.1 | RadC family protein | - |
| H9203_RS22140 (4504808) | 4504808..4505281 | - | 474 | WP_000855059.1 | antirestriction protein | - |
| H9203_RS22145 (4505621) | 4505621..4506439 | - | 819 | WP_042631095.1 | DUF932 domain-containing protein | - |
| H9203_RS22150 (4506557) | 4506557..4506752 | - | 196 | Protein_4331 | DUF905 family protein | - |
| H9203_RS22155 (4506823) | 4506823..4507014 | - | 192 | Protein_4332 | autotransporter domain-containing protein | - |
| H9203_RS22160 (4507098) | 4507098..4508120 | + | 1023 | WP_000255946.1 | IS21-like element IS100 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | blaCTX-M-55 | cheD / fimH / fimG / fimF / fimD / fimC / fimI / fimA / fimE / fimB | 4339383..4508500 | 169117 | |
| - | flank | IS/Tn | - | - | 4499423..4500964 | 1541 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14144.26 Da Isoelectric Point: 7.3249
>T171486 WP_000854759.1 NZ_CP060914:c4503411-4503034 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR
Download Length: 378 bp
>T171486 NZ_CP077931:2453342-2453551 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG
Antitoxin
Download Length: 123 a.a. Molecular weight: 13592.40 Da Isoelectric Point: 6.6255
>AT171486 WP_001295723.1 NZ_CP060914:c4503869-4503501 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK
Download Length: 369 bp
>AT171486 NZ_CP077931:2453037-2453330 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0J2AEA6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1NM52 |