Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4422045..4422621 Replicon chromosome
Accession NZ_CP060914
Organism Escherichia coli strain EC18

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag H9203_RS21735 Protein ID WP_001131965.1
Coordinates 4422334..4422621 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag H9203_RS21730 Protein ID WP_019842999.1
Coordinates 4422045..4422347 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9203_RS21710 (4417658) 4417658..4418170 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
H9203_RS21715 (4418560) 4418560..4420710 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
H9203_RS21720 (4420841) 4420841..4421044 + 204 WP_000467859.1 YbdD/YjiX family protein -
H9203_RS21725 (4421055) 4421055..4422011 + 957 WP_001295525.1 GTPase -
H9203_RS21730 (4422045) 4422045..4422347 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
H9203_RS21735 (4422334) 4422334..4422621 - 288 WP_001131965.1 BrnT family toxin Toxin
H9203_RS21740 (4422818) 4422818..4423984 + 1167 WP_000800825.1 restriction endonuclease -
H9203_RS21745 (4424048) 4424048..4425667 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaCTX-M-55 cheD / fimH / fimG / fimF / fimD / fimC / fimI / fimA / fimE / fimB 4339383..4508500 169117
- flank IS/Tn - - 4425786..4426163 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T171485 WP_001131965.1 NZ_CP060914:c4422621-4422334 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T171485 NZ_CP077931:c979260-979009 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGATACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGATCTTGATGAAGCAGTAGGTATTATGGTAAGTCAAGTTGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT171485 WP_019842999.1 NZ_CP060914:c4422347-4422045 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT171485 NZ_CP077931:c979019-978846 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References