Detailed information of TA system TA171483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3805568..3806405
• Replicon: chromosome
• Accession: NZ_CP060914
• Organism: Escherichia coli strain EC18

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: H9203_RS18700
• Protein ID: WP_000227784.1
• Coordinates: 3805863..3806405 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: H9203_RS18695
• Protein ID: WP_001297137.1
• Coordinates: 3805568..3805879 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9203_RS18670 (3800588)	3800588..3801535	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
H9203_RS18675 (3801557)	3801557..3803548	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
H9203_RS18680 (3803538)	3803538..3804152	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
H9203_RS18685 (3804152)	3804152..3804481	+	330	WP_072649523.1	cytochrome o ubiquinol oxidase subunit IV	-
H9203_RS18690 (3804493)	3804493..3805383	+	891	WP_000971336.1	heme o synthase	-
H9203_RS18695 (3805568)	3805568..3805879	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
H9203_RS18700 (3805863)	3805863..3806405	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
H9203_RS18705 (3806461)	3806461..3807396	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
H9203_RS18710 (3807804)	3807804..3809168	+	1365	WP_001000974.1	MFS transporter	-
H9203_RS18715 (3809296)	3809296..3809787	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
H9203_RS18720 (3809955)	3809955..3810866	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T171483 WP_000227784.1 NZ_CP060914:3805863-3806405 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T171483 NZ_CP077931:917042-917230 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT171483 WP_001297137.1 NZ_CP060914:3805568-3805879 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT171483 NZ_CP077931:916753-917013 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTACCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3