Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3771492..3772110 Replicon chromosome
Accession NZ_CP060914
Organism Escherichia coli strain EC18

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9203_RS18530 Protein ID WP_001291435.1
Coordinates 3771892..3772110 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9203_RS18525 Protein ID WP_000344800.1
Coordinates 3771492..3771866 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9203_RS18515 (3766581) 3766581..3767774 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9203_RS18520 (3767797) 3767797..3770946 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9203_RS18525 (3771492) 3771492..3771866 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9203_RS18530 (3771892) 3771892..3772110 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9203_RS18535 (3772281) 3772281..3772832 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9203_RS18540 (3772948) 3772948..3773418 + 471 WP_000136192.1 YlaC family protein -
H9203_RS18545 (3773582) 3773582..3775132 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9203_RS18550 (3775174) 3775174..3775527 - 354 WP_000878141.1 DUF1428 family protein -
H9203_RS18560 (3775906) 3775906..3776217 + 312 WP_000409911.1 MGMT family protein -
H9203_RS18565 (3776248) 3776248..3776820 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171482 WP_001291435.1 NZ_CP060914:3771892-3772110 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171482 NZ_CP077930:18084-18335 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGATACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGATCTTGATGAAGCAGTAGGTATTATGGTAAGTCAAGTTGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171482 WP_000344800.1 NZ_CP060914:3771492-3771866 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171482 NZ_CP077930:18325-18498 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References