Detailed information of TA system TA171470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1642755..1643380
• Replicon: chromosome
• Accession: NZ_CP060914
• Organism: Escherichia coli strain EC18

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9203_RS08175
• Protein ID: WP_000911330.1
• Coordinates: 1642982..1643380 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H9203_RS08170
• Protein ID: WP_000450524.1
• Coordinates: 1642755..1642982 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9203_RS08145 (1638558)	1638558..1639028	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H9203_RS08150 (1639028)	1639028..1639600	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H9203_RS08155 (1639746)	1639746..1640624	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H9203_RS08160 (1640641)	1640641..1641675	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
H9203_RS08165 (1641888)	1641888..1642601	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H9203_RS08170 (1642755)	1642755..1642982	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H9203_RS08175 (1642982)	1642982..1643380	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9203_RS08180 (1643527)	1643527..1644390	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
H9203_RS08185 (1644405)	1644405..1646420	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
H9203_RS08190 (1646494)	1646494..1647192	+	699	WP_000679812.1	esterase	-
H9203_RS08195 (1647273)	1647273..1647473	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T171470 WP_000911330.1 NZ_CP060914:1642982-1643380 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T171470 NZ_CP077926:1321123-1321299 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT171470 WP_000450524.1 NZ_CP060914:1642755-1642982 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT171470 NZ_CP077926:c1321461-1321287 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33