Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1190576..1191230 Replicon chromosome
Accession NZ_CP060914
Organism Escherichia coli strain EC18

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H9203_RS05940 Protein ID WP_000244772.1
Coordinates 1190823..1191230 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9203_RS05935 Protein ID WP_000354046.1
Coordinates 1190576..1190842 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9203_RS05910 (1185864) 1185864..1186607 + 744 WP_000951964.1 SDR family oxidoreductase -
H9203_RS05915 (1186664) 1186664..1188097 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
H9203_RS05920 (1188142) 1188142..1188453 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
H9203_RS05925 (1188617) 1188617..1189276 + 660 WP_000250274.1 hemolysin III family protein -
H9203_RS05930 (1189353) 1189353..1190333 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9203_RS05935 (1190576) 1190576..1190842 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9203_RS05940 (1190823) 1190823..1191230 + 408 WP_000244772.1 protein YgfX Toxin
H9203_RS05945 (1191270) 1191270..1191791 - 522 WP_001055874.1 flavodoxin FldB -
H9203_RS05950 (1191903) 1191903..1192799 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9203_RS05955 (1192824) 1192824..1193534 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9203_RS05960 (1193540) 1193540..1195273 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T171468 WP_000244772.1 NZ_CP060914:1190823-1191230 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171468 NZ_CP077926:1220186-1220395 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171468 WP_000354046.1 NZ_CP060914:1190576-1190842 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171468 NZ_CP077926:1219881-1220174 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References