Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 513156..513787 Replicon chromosome
Accession NZ_CP060914
Organism Escherichia coli strain EC18

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H9203_RS02455 Protein ID WP_001259388.1
Coordinates 513156..513431 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H9203_RS02460 Protein ID WP_000593555.1
Coordinates 513428..513787 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9203_RS02440 (508160) 508160..509227 + 1068 WP_000361485.1 HlyD family secretion protein -
H9203_RS02445 (509224) 509224..511959 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
H9203_RS02450 (511959) 511959..513083 + 1125 WP_001314210.1 ABC transporter permease -
H9203_RS02455 (513156) 513156..513431 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H9203_RS02460 (513428) 513428..513787 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H9203_RS02465 (513907) 513907..514308 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H9203_RS02470 (514314) 514314..515120 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H9203_RS02475 (515117) 515117..515881 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H9203_RS02480 (515881) 515881..516714 - 834 WP_038999760.1 nickel ABC transporter permease subunit NikC -
H9203_RS02485 (516711) 516711..517655 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T171464 WP_001259388.1 NZ_CP060914:513156-513431 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T171464 NZ_CP077925:c2005123-2004971 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGAGAGTCAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT171464 WP_000593555.1 NZ_CP060914:513428-513787 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT171464 NZ_CP077925:c2005747-2005148 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
CAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References