Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 66170..66813 Replicon plasmid pEC19-2
Accession NZ_CP060909
Organism Escherichia coli strain EC19

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H9204_RS23925 Protein ID WP_001034044.1
Coordinates 66397..66813 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H9204_RS23920 Protein ID WP_001261286.1
Coordinates 66170..66400 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9204_RS23900 (62801) 62801..63556 - 756 WP_000852146.1 replication initiation protein RepE -
H9204_RS23905 (64278) 64278..65084 - 807 WP_000016970.1 site-specific integrase -
H9204_RS23910 (65085) 65085..65390 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H9204_RS23915 (65392) 65392..65610 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H9204_RS23920 (66170) 66170..66400 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9204_RS23925 (66397) 66397..66813 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H9204_RS23930 (66888) 66888..68453 + 1566 WP_001128474.1 AAA family ATPase -
H9204_RS23935 (68438) 68438..69460 + 1023 WP_000361402.1 helicase UvrD -
H9204_RS23940 (69714) 69714..70400 - 687 Protein_84 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..88659 88659


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T171458 WP_001034044.1 NZ_CP060909:66397-66813 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T171458 NZ_CP077924:693900-694202 [Staphylococcus aureus]
ATGGAAGCAAAAGTAATGGCAAGATACGTTGTATTGATTTTAGCGTTAGTCAATCAGTTTTTAGCGAATAAAGGTATAAG
CCCGATACCAGTAGATGAAGAAAGTATTTCATCGATCATCTTAACAGTTATTGCTTTATACACTACGTATAAAGACAATC
CAACATCACAAGAAGGACGTTGGGCGAATCAAAAATTAAAGAAATATAAAGCAGAAAGTAAATATAGAAAAGCAACAGGA
CAAGCACCTATCAAAGAAGTGATGACACCTACAAATATGAACGACACAAATGATTTAGGGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT171458 WP_001261286.1 NZ_CP060909:66170-66400 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT171458 NZ_CP077924:c693820-693754 [Staphylococcus aureus]
ATAAATAGAAAAAGGGCAACATGCGGAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References