Detailed information of TA system TA171441

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1877551..1878383
• Replicon: chromosome
• Accession: NZ_CP060908
• Organism: Escherichia coli strain EC19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: H9204_RS08965
• Protein ID: WP_001514886.1
• Coordinates: 1877551..1877925 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: H9204_RS08970
• Protein ID: WP_001360327.1
• Coordinates: 1878015..1878383 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9204_RS08930 (1873067)	1873067..1873540	+	474	WP_001105385.1	DNA gyrase inhibitor SbmC	-
H9204_RS08935 (1873739)	1873739..1874797	+	1059	WP_032181141.1	FUSC family protein	-
H9204_RS08940 (1874969)	1874969..1875298	+	330	WP_000450409.1	DUF496 family protein	-
H9204_RS08945 (1875399)	1875399..1875665	-	267	WP_063121129.1	EutP/PduV family microcompartment system protein	-
H9204_RS08950 (1876035)	1876035..1876406	-	372	WP_001295631.1	IS110 family transposase	-
H9204_RS08955 (1877222)	1877222..1877302	-	81	Protein_1760	hypothetical protein	-
H9204_RS08960 (1877402)	1877402..1877554	-	153	Protein_1761	DUF5983 family protein	-
H9204_RS08965 (1877551)	1877551..1877925	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
H9204_RS08970 (1878015)	1878015..1878383	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9204_RS08975 (1878546)	1878546..1878767	-	222	WP_000692286.1	DUF987 domain-containing protein	-
H9204_RS08980 (1878830)	1878830..1879306	-	477	WP_001186774.1	RadC family protein	-
H9204_RS08985 (1879322)	1879322..1879795	-	474	WP_000855059.1	antirestriction protein	-
H9204_RS08990 (1880137)	1880137..1880955	-	819	WP_088540043.1	DUF932 domain-containing protein	-
H9204_RS08995 (1881073)	1881073..1881268	-	196	Protein_1768	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T171441 WP_001514886.1 NZ_CP060908:c1877925-1877551 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T171441 NZ_CP077922:c1472823-1472461 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT171441 WP_001360327.1 NZ_CP060908:c1878383-1878015 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT171441 NZ_CP077922:c1472990-1472820 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3

Antitoxin

• AlphaFold DB: A0A7U9IW59