Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 911158..911812 Replicon chromosome
Accession NZ_CP060908
Organism Escherichia coli strain EC19

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H9204_RS04445 Protein ID WP_000244777.1
Coordinates 911405..911812 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9204_RS04440 Protein ID WP_000354046.1
Coordinates 911158..911424 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9204_RS04415 (906446) 906446..907189 + 744 Protein_870 SDR family oxidoreductase -
H9204_RS04420 (907246) 907246..908679 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H9204_RS04425 (908724) 908724..909035 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
H9204_RS04430 (909199) 909199..909858 + 660 WP_000250274.1 hemolysin III family protein -
H9204_RS04435 (909935) 909935..910915 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9204_RS04440 (911158) 911158..911424 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9204_RS04445 (911405) 911405..911812 + 408 WP_000244777.1 protein YgfX Toxin
H9204_RS04450 (911852) 911852..912373 - 522 WP_001055874.1 flavodoxin FldB -
H9204_RS04455 (912485) 912485..913381 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9204_RS04460 (913406) 913406..914116 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9204_RS04465 (914122) 914122..915855 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T171436 WP_000244777.1 NZ_CP060908:911405-911812 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T171436 NZ_CP077921:2597962-2598324 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT171436 WP_000354046.1 NZ_CP060908:911158-911424 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT171436 NZ_CP077921:2597795-2597965 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References