Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 65095..65620 Replicon plasmid pEC20-2
Accession NZ_CP060903
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H9205_RS23910 Protein ID WP_001159871.1
Coordinates 65095..65400 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H9205_RS23915 Protein ID WP_000813630.1
Coordinates 65402..65620 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9205_RS23895 (61057) 61057..62223 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
H9205_RS23900 (62811) 62811..63566 - 756 WP_000852146.1 replication initiation protein RepE -
H9205_RS23905 (64288) 64288..65094 - 807 WP_000016970.1 site-specific integrase -
H9205_RS23910 (65095) 65095..65400 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H9205_RS23915 (65402) 65402..65620 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H9205_RS23920 (66180) 66180..66410 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
H9205_RS23925 (66407) 66407..66823 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H9205_RS23930 (66898) 66898..68463 + 1566 WP_001128474.1 AAA family ATPase -
H9205_RS23935 (68448) 68448..69470 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..88680 88680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T171426 WP_001159871.1 NZ_CP060903:c65400-65095 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T171426 NZ_CP077920:1563259-1563525 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT171426 WP_000813630.1 NZ_CP060903:c65620-65402 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT171426 NZ_CP077920:1563008-1563259 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References