Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4241413..4241910 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9206_RS20645 Protein ID WP_001173343.1
Coordinates 4241413..4241586 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9206_RS20650 Protein ID WP_000398619.1
Coordinates 4241614..4241910 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS20635 (4238083) 4238083..4239540 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H9206_RS20640 (4239777) 4239777..4241294 + 1518 WP_001295074.1 lysine--tRNA ligase -
H9206_RS20645 (4241413) 4241413..4241586 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9206_RS20650 (4241614) 4241614..4241910 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9206_RS20655 (4242137) 4242137..4242409 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
H9206_RS20660 (4242421) 4242421..4242651 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
H9206_RS20665 (4242832) 4242832..4244463 + 1632 WP_001216487.1 sensor histidine kinase -
H9206_RS20670 (4244460) 4244460..4245179 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T171396 WP_001173343.1 NZ_CP060899:c4241586-4241413 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T171396 NZ_CP077915:2522098-2522460 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT171396 WP_000398619.1 NZ_CP060899:c4241910-4241614 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT171396 NZ_CP077915:2521931-2522101 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References