Detailed information of TA system TA171394

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4061168..4062003
• Replicon: chromosome
• Accession: NZ_CP060899
• Organism: Escherichia coli strain EC21

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: H9206_RS19695
• Protein ID: WP_000854759.1
• Coordinates: 4061168..4061545 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: H9206_RS19700
• Protein ID: WP_001295723.1
• Coordinates: 4061635..4062003 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9206_RS19670 (4057557)	4057557..4059098	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
H9206_RS19675 (4059692)	4059692..4059868	-	177	Protein_3852	helix-turn-helix domain-containing protein	-
H9206_RS19680 (4060235)	4060235..4060384	-	150	Protein_3853	hypothetical protein	-
H9206_RS19685 (4060490)	4060490..4060666	-	177	WP_000839286.1	DUF957 domain-containing protein	-
H9206_RS19690 (4060683)	4060683..4061171	-	489	WP_000761690.1	DUF5983 family protein	-
H9206_RS19695 (4061168)	4061168..4061545	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
H9206_RS19700 (4061635)	4061635..4062003	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9206_RS19705 (4062166)	4062166..4062387	-	222	WP_000692345.1	DUF987 domain-containing protein	-
H9206_RS19710 (4062450)	4062450..4062926	-	477	WP_001186775.1	RadC family protein	-
H9206_RS19715 (4062942)	4062942..4063415	-	474	WP_000855059.1	antirestriction protein	-
H9206_RS19720 (4063755)	4063755..4064573	-	819	WP_042631095.1	DUF932 domain-containing protein	-
H9206_RS19725 (4064691)	4064691..4064886	-	196	Protein_3862	DUF905 family protein	-
H9206_RS19730 (4064957)	4064957..4065982	-	1026	Protein_3863	autotransporter domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4047621..4105575	57954
	-	flank	IS/Tn	-	-	4057557..4059098	1541

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T171394 WP_000854759.1 NZ_CP060899:c4061545-4061168 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T171394 NZ_CP077915:2188280-2188546 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT171394 WP_001295723.1 NZ_CP060899:c4062003-4061635 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT171394 NZ_CP077915:2188029-2188280 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52