171391

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3436213..3437050 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag H9206_RS16705 Protein ID WP_000227784.1
Coordinates 3436508..3437050 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag H9206_RS16700 Protein ID WP_001297137.1
Coordinates 3436213..3436524 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS16675 (3431233) 3431233..3432180 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
H9206_RS16680 (3432202) 3432202..3434193 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H9206_RS16685 (3434183) 3434183..3434797 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H9206_RS16690 (3434797) 3434797..3435126 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
H9206_RS16695 (3435138) 3435138..3436028 + 891 WP_000971336.1 heme o synthase -
H9206_RS16700 (3436213) 3436213..3436524 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
H9206_RS16705 (3436508) 3436508..3437050 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
H9206_RS16710 (3437106) 3437106..3438041 - 936 WP_001365761.1 tetratricopeptide repeat protein -
H9206_RS16715 (3438449) 3438449..3439813 + 1365 WP_001000974.1 MFS transporter -
H9206_RS16720 (3439941) 3439941..3440432 - 492 WP_001138904.1 nucleotide binding protein YajQ -
H9206_RS16725 (3440600) 3440600..3441511 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T171391 WP_000227784.1 NZ_CP060899:3436508-3437050 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T171391 NZ_CP077911:c2617216-2617109 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT171391 WP_001297137.1 NZ_CP060899:3436213-3436524 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT171391 NZ_CP077911:2617032-2617092 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References