Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3402137..3402755 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H9206_RS16535 Protein ID WP_001291435.1
Coordinates 3402537..3402755 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H9206_RS16530 Protein ID WP_000344800.1
Coordinates 3402137..3402511 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS16520 (3397226) 3397226..3398419 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H9206_RS16525 (3398442) 3398442..3401591 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H9206_RS16530 (3402137) 3402137..3402511 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H9206_RS16535 (3402537) 3402537..3402755 + 219 WP_001291435.1 HHA domain-containing protein Toxin
H9206_RS16540 (3402926) 3402926..3403477 + 552 WP_000102564.1 maltose O-acetyltransferase -
H9206_RS16545 (3403593) 3403593..3404063 + 471 WP_000136192.1 YlaC family protein -
H9206_RS16550 (3404227) 3404227..3405777 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H9206_RS16555 (3405819) 3405819..3406172 - 354 WP_000878141.1 DUF1428 family protein -
H9206_RS16565 (3406551) 3406551..3406862 + 312 WP_000409911.1 MGMT family protein -
H9206_RS16570 (3406893) 3406893..3407465 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T171390 WP_001291435.1 NZ_CP060899:3402537-3402755 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T171390 NZ_CP077911:c2591996-2591730 [Staphylococcus aureus]
ATGGTTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTAATTAAAAGTATTGACAGAAATGGTGCGTTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT171390 WP_000344800.1 NZ_CP060899:3402137-3402511 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT171390 NZ_CP077911:c2592247-2591996 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATAGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTGTAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References